We offer different solutions to meet every reasearcher’s need; we perform multiparameter assays based on Luminex technology as well as single biomarker based on ELISA.
Biomarker Immunoassays service offered by Labospace is certified by internally generated ISO regulatory SOPS. Our services are available on every commercial kit. The required sample volumes start from 25- 50uL and allow researchers to collect more data in less time than other assays. Researchers can generate highly efficient multiplex assays selecting up to 50 analytes available within Labospace list which contains more than 330 biomarkers of various species (human, mouse, rat, porcine, canine). Multiparameter Flow Cytometry is also available where Fluorescent dyes or fluorophore bearing specific antibodies (up to 10 different combination) can be used to stain cellular surface structures, intracellular molecules or nuclear components of cells.
Multiparameter Luminex
In multiplex bead array assays (MBAA), beads of discrete fluorescence intensities and wavelengths provide a capture surface for specific analytes enabling detection of multiple analytes in a single sample. Bead based assays of analyte is an attractive strategy for obtaining large numbers of measurements rapidly. MBAA is an open access system; namely, the parameters to be measured can be determined by the user. The variety of source increases its area of application (nucleic acids, antigens, antibodies, receptors). Since the detection method is based on flow cytometry and this allows repeatability, MBAA performs the quality control of the measurement, simultaneously with the measurement itself. The MBAA readers allow analysis of up to 100 reactions or assays per well. This remarkable capability allows incorporating a large number of assays, such as DNA, receptor ligand, immunoassay and enzyme in life science research, drug discovery as well as diagnostic areas with the reduction of worktime and costs over traditional methods such as ELISA, western blotting, PCR, and traditional arrays.
The principles of the measurement
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Color coded beads, pre-coated with analyte specific capture antibody for the molecule of interest are added.
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Analyte specific antibodies capture the analyte of interest. Biotinylated detection antibodies specific to the analyte of interest are added and form an antibody antigen sandwich.
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Phycoerythrin (PE) conjugated Streptavidin is added.
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The beads are read by a dual laser flow based detection instrument which excites the internal dyes marking the beads set and a second laser excites PE, the fluorescent dye on the reporter molecule.
Multiparameter Flow Cytometry
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Lorem ipsum dolor sit amet, facer oratio nec ei, senserit inciderint ei sit. Qui dicat mazim ut. Mei dicunt salutandi ei, commune democritum duo eu. Oratio rationibus disputationi ex nec, an per prima timeam. Vix elitr reprimique no, clita comprehensam pro et, sit odio ferri euismod et. Putant nusquam mnesarchum sea ut.
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Lorem ipsum dolor sit amet, facer oratio nec ei, senserit inciderint ei sit. Qui dicat mazim ut. Mei dicunt salutandi ei, commune democritum duo eu. Oratio rationibus disputationi ex nec, an per prima timeam. Vix elitr reprimique no, clita comprehensam pro et, sit odio ferri euismod et. Putant nusquam mnesarchum sea ut.
Single biomarker on ELISA
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Lorem ipsum dolor sit amet, facer oratio nec ei, senserit inciderint ei sit. Qui dicat mazim ut. Mei dicunt salutandi ei, commune democritum duo eu.
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Lorem ipsum dolor sit amet, facer oratio nec ei, senserit inciderint ei sit. Qui dicat mazim ut. Mei dicunt salutandi ei, commune democritum duo eu. Oratio rationibus disputationi ex nec, an per prima timeam. Vix elitr reprimique no, clita comprehensam pro et, sit odio ferri euismod et. Putant nusquam mnesarchum sea ut.
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Lorem ipsum dolor sit amet, facer oratio nec ei, senserit inciderint ei sit. Qui dicat mazim ut. Mei dicunt salutandi ei, commune democritum duo eu. Oratio rationibus disputationi ex nec, an per prima timeam. Vix elitr reprimique no, clita comprehensam pro et, sit odio ferri euismod et. Putant nusquam mnesarchum sea ut.